Dido1^tm2Cmar
Targeted Allele Detail

Summary

• Symbol: Dido1^tm2Cmar
• Name: death inducer-obliterator 1; targeted mutation 2, Carlos Martinez-A
• MGI ID: MGI:5318886
• Synonyms: Dido3deltaCT-RFP
• Gene: Dido1 Location: Chr2:180299757-180351792 bp, - strand Genetic Position: Chr2, Syntenic
• Alliance: Dido1^tm2Cmar page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183346
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 16 was replaced with an in frame RFP cassette and an FRT flanked neo cassette via homologous recombination. This disrupts the Dido3 isoform while leaving the other 2 isoforms intact. RT-PCR confirmed the absence of expression of isoform 3 and the presence of isoforms 1 and 2 in homozygous embryos. (J:183346)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dido1 Mutation: 186 strains or lines available

References

• Original: J:183346 Futterer A, et al., Ablation of Dido3 compromises lineage commitment of stem cells in vitro and during early embryonic development. Cell Death Differ. 2012 Jan;19(1):132-43
• All: 2 reference(s)