Pmch^{tm1.1(HBEGF)Rpa}
Targeted Allele Detail

Summary

• Symbol: Pmch^{tm1.1(HBEGF)Rpa}
• Name: pro-melanin-concentrating hormone; targeted mutation 1.1, Richard D Palmiter
• MGI ID: MGI:5319632
• Synonyms: Pmch^DTR
• Gene: Pmch Location: Chr10:87926934-87928236 bp, + strand Genetic Position: Chr10, 43.7 cM, cytoband C2
• Alliance: Pmch^{tm1.1(HBEGF)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:181947
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Pmch^{tm1.1(HBEGF)Rpa} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Mutation details: The human diphtheria toxin receptor followed by an IRES-EGFP reporter gene and an FRT flanked neo cassette replaced parts of the first and second coding exons via homologous recombination. Flp mediated recombination removed the neo cassette. (J:181947)

Schematic of the Pmch^{tm1.1(HBEGF)Rpa} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pmch Mutation: 27 strains or lines available

References

• Original: J:181947 Wu Q, et al., Ablation of neurons expressing agouti-related protein, but not melanin concentrating hormone, in leptin-deficient mice restores metabolic functions and fertility. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3155-60
• All: 2 reference(s)