Cdk2^tm1.1Jro
Targeted Allele Detail

Summary

• Symbol: Cdk2^tm1.1Jro
• Name: cyclin dependent kinase 2; targeted mutation 1.1, James Roberts
• MGI ID: MGI:5319654
• Synonyms: CDK2AF, CDK2^{T14A Y15F}
• Gene: Cdk2 Location: Chr10:128533808-128540900 bp, - strand Genetic Position: Chr10, 77.13 cM, cytoband D3
• Alliance: Cdk2^tm1.1Jro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183801
• Parent Cell Line: AK7.1 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 1 was replaced with one in which nucleotide substitutions resulted in the amino acid substitution of alanine for threonine at position 14 (T14A) and phenylalanine for tyrosine at position 15 (Y15F). Cre-mediated recombination removed a floxed neo cassette inserted downstream of the modified exon 1. (J:183801)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk2 Mutation: 18 strains or lines available

References

• Original: J:183801 Zhao H, et al., Loss of cyclin-dependent kinase 2 (CDK2) inhibitory phosphorylation in a CDK2AF knock-in mouse causes misregulation of DNA replication and centrosome duplication. Mol Cell Biol. 2012 Apr;32(8):1421-32
• All: 1 reference(s)