Mir721tm1Wtsi
Targeted Allele Detail
|
Symbol: |
Mir721tm1Wtsi |
Name: |
microRNA 721; targeted mutation 1, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5320076 |
Synonyms: |
Mir721tm1(mirKO)Wtsi |
Gene: |
Mir721 Location: Chr5:136404570-136404657 bp, - strand Genetic Position: Chr5, 75.99 cM
|
|
Mutant Cell Lines: |
mirKO_ES_PuDtk_12D7, mirKO_ES_PuDtk_12D8, mirKO_ES_PuDtk_12D9, mirKO_ES_PuDtk_12D10, mirKO_ES_PuDtk_12D11, mirKO_ES_PuDtk_12D12 |
Germline Transmission: |
Unknown
|
Parent Cell Line: |
JM8A3 (ES Cell)
|
Strain of Origin: |
C57BL/6N-Atm1Brd
|
Project Collection: |
Sanger miRNA knockouts
|
|
Allele Type: |
|
Targeted (Null/knockout) |
Mutations: |
|
Insertion, Intragenic deletion
Vector: PGK_EM7_PuDtk_bGHpA
|
|
|
Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 204 starting at position 136404512 and ending at position 136404716 of Chromosome 5 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site.
(J:174268)
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Mir721 Mutation: |
1 strain or line available
|
|
Original: |
J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5 |
All: |
1 reference(s) |
|