Piwil2^tm1.1Doca
Targeted Allele Detail

Summary

• Symbol: Piwil2^tm1.1Doca
• Name: piwi-like RNA-mediated gene silencing 2; targeted mutation 1.1, Donal O'Carroll
• MGI ID: MGI:5320635
• Synonyms: Mili^D813A, Mili^DAH
• Gene: Piwil2 Location: Chr14:70609926-70666832 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Piwil2^tm1.1Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184034
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 21 was replaced with a modified exon in which nucleotide substitutions (GAT to GCT) results in the amino acid substitution of alanine for aspartic acid at position 813 (D813A) and an FRT-flanked neo cassette. Flp-mediated recombination removed the neo cassette. (J:184034)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piwil2 Mutation: 54 strains or lines available

References

• Original: J:184034 De Fazio S, et al., The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature. 2011 Dec 8;480(7376):259-63
• All: 3 reference(s)