Piwil4^tm1.1Doca
Targeted Allele Detail

Summary

• Symbol: Piwil4^tm1.1Doca
• Name: piwi-like RNA-mediated gene silencing 4; targeted mutation 1.1, Donal O'Carroll
• MGI ID: MGI:5320636
• Synonyms: Miwi2^D761A, Miwi2^DAH
• Gene: Piwil4 Location: Chr9:14613072-14651968 bp, - strand Genetic Position: Chr9, 4.25 cM
• Alliance: Piwil4^tm1.1Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184034
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 17 was replaced with a modified exon in which nucleotide substitutions (GAC to GCT) result in the amino acid substitution of alanine for aspartic acid at position 761 (D761A). An FRT-flanked neo cassette was also inserted but Flp-mediated recombination removed the neo cassette. (J:184034)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piwil4 Mutation: 49 strains or lines available

References

• Original: J:184034 De Fazio S, et al., The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature. 2011 Dec 8;480(7376):259-63
• All: 2 reference(s)