Piwil4^tm2.1Doca
Targeted Allele Detail

Summary

• Symbol: Piwil4^tm2.1Doca
• Name: piwi-like RNA-mediated gene silencing 4; targeted mutation 2.1, Donal O'Carroll
• MGI ID: MGI:5320638
• Synonyms: Miwi2^-
• Gene: Piwil4 Location: Chr9:14613072-14651968 bp, - strand Genetic Position: Chr9, 4.25 cM
• Alliance: Piwil4^tm2.1Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184034
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 17. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 17. Cre-mediated recombintion removed exon 17 and the neo cassette. Immunohistochemistry confirmed the absence of protein expression in the fetal testis. (J:184034)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piwil4 Mutation: 49 strains or lines available

References

• Original: J:184034 De Fazio S, et al., The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature. 2011 Dec 8;480(7376):259-63
• All: 2 reference(s)