Egfr^tm1.1Tyj
Targeted Allele Detail

Summary

• Symbol: Egfr^tm1.1Tyj
• Name: epidermal growth factor receptor; targeted mutation 1.1, Tyler Jacks
• MGI ID: MGI:5320891
• Synonyms: Egfr^{tm1.1(L858R)Tyj}
• Gene: Egfr Location: Chr11:16702203-16868158 bp, + strand Genetic Position: Chr11, 9.41 cM, cytoband A1-A4
• Alliance: Egfr^tm1.1Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: V26.2 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Single point mutation
• Mutation details: The targeting construct introduced an FRT site flanked neomycin resistance gene cassette into intron 20 and a T->G point mutation in exon 21, changing leucine codon 860 (CTG) to arginine (CGG) (p.L860R). The locus was re-targeted to place a lox-Stop-lox (LSL) cassette in intron 1. Flp-mediated recombination removed the neo cassette. The mutation is the equivalent of the human p.L858R mutation associated with lung cancer susceptibility. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Egfr Mutation: 87 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 3 reference(s)