Gt(ROSA)26Sor^{tm4(EEF1A1-EGFP)Npa}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(EEF1A1-EGFP)Npa}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Novartis Pharma AG
• MGI ID: MGI:5424000
• Synonyms: mR26-EF1alpha-EGFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(EEF1A1-EGFP)Npa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184312
• Parent Cell Line: BALB/c-I (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

Allele Type: Targeted (RMCE-ready, Reporter) Mutation: Insertion Mutation details: Recombinase-mediated cassette exchange (RMCE) replaced the floxed hygro cassette in Gt(ROSA)26Sor^tm1Npa with an EEF1A1 promoter, EGFP and FRT-flanked neo cassette. EGFP is detected in all organs analyzed (salivary glands, heart, pancreas, liver, kidney, fat, muscle, brain and testis) and strong levels in testis. (J:184312)

Generation of the Gt(ROSA)26Sor^tm2(CAG-EGFP)Npa, Gt(ROSA)26Sor^tm3(CMV-EGFP)Npa, and Gt(ROSA)26Sor^tm4(EEF1A1-EGFP)Npa alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:184312 Tchorz JS, et al., A modified RMCE-compatible Rosa26 locus for the expression of transgenes from exogenous promoters. PLoS One. 2012;7(1):e30011
• All: 1 reference(s)