Col2a1^tm1.1Mcali
Targeted Allele Detail

Summary

• Symbol: Col2a1^tm1.1Mcali
• Name: collagen, type II, alpha 1; targeted mutation 1.1, Audrey McAlinden
• MGI ID: MGI:5425899
• Synonyms: Col2a1^+ex2
• Gene: Col2a1 Location: Chr15:97873483-97902525 bp, - strand Genetic Position: Chr15, 53.97 cM
• Alliance: Col2a1^tm1.1Mcali page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184499
• Parent Cell Line: EDJ22 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with an exon 2 in which nucleotide substitutions (ACT GCC AGT Ggtcgta to ACG GCC AGT Ggtaagt) resulted in a silent mutation to produce an HaeIII site and mutate the exon IIa 5' splice site. Cre-mediated recombination removed a floxed neo cassette inserted downstream of the modified exon 2. Mice only express isoform IIa. (J:184499)

Expression

In Mice Carrying this Mutation:

92 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col2a1 Mutation: 69 strains or lines available

References

• Original: J:184499 Lewis R, et al., Disruption of the developmentally-regulated Col2a1 pre-mRNA alternative splicing switch in a transgenic knock-in mouse model. Matrix Biol. 2012 Apr;31(3):214-26
• All: 1 reference(s)