Lrp2^tm3Tew
Targeted Allele Detail

Summary

• Symbol: Lrp2^tm3Tew
• Name: low density lipoprotein receptor-related protein 2; targeted mutation 3, Thomas E Willnow
• MGI ID: MGI:5426261
• Synonyms: Megalin^TgICD
• Gene: Lrp2 Location: Chr2:69254679-69416373 bp, - strand Genetic Position: Chr2, 40.74 cM
• Alliance: Lrp2^tm3Tew page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184259
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 and 3.6Kb of intron 1 were replaced by a minigene construct encoding amino acids 4448-4661 of the mouse Lrp2 (megalin) polypeptide (NM_001081088) and stop codon fused to 3 copies of the flag epitope at the amino terminus. A neo resistance cassette immediately follows the megalin intracellular domain (ICD) construct. Western blot analysis confirmed cytosolic expression of the ICD form in kidneys but not the wild-type product. (J:184259)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lrp2 Mutation: 261 strains or lines available

References

• Original: J:184259 Christ A, et al., The soluble intracellular domain of megalin does not affect renal proximal tubular function in vivo. Kidney Int. 2010 Sep;78(5):473-7
• All: 1 reference(s)