Gt(ROSA)26Sor^{tm1.1(Maml1/EGFP)Hri}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Maml1/EGFP)Hri}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Palle Serup
• MGI ID: MGI:5426507
• Synonyms: R26^dnMaml1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Maml1/EGFP)Hri} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184814
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Dominant negative, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(Maml1/EGFP)Hri} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Maml1 (MGI:1890504)

• Mutation details: Homologous recombination in E14 ES cells introduced a dominant negative Mastermind-like 1/EGFP fusion sequence ((Maml1/EGFP) into the Gt(ROSA)26Sor locus. An Frt-flanked neo cassette was also introduced with the targeting construct, and was subsequently deleted from germline chimeras by breeding them with Flpe-expressing mice. The dnMaml1 fusion protein is a potent inhibitor of the 4 mammalian Notch receptors. (J:184814)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:184814 Horn S, et al., Mind bomb 1 is required for pancreatic beta-cell formation. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7356-61
• All: 6 reference(s)