Myl2^tm2.1Chen
Targeted Allele Detail

Summary

• Symbol: Myl2^tm2.1Chen
• Name: myosin, light polypeptide 2, regulatory, cardiac, slow; targeted mutation 2.1, Ju Chen
• MGI ID: MGI:5426525
• Synonyms: Myl2^DM
• Gene: Myl2 Location: Chr5:122239014-122251535 bp, + strand Genetic Position: Chr5, Syntenic
• Alliance: Myl2^tm2.1Chen page

Dilated cardiomyopathy in Myl2^tm2.1Chen/Myl2^tm2.1Chen mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184548
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: Nucleotide substitutions AG to GC in codon 14 and T to G in codon 15 resulted in the amino acid substitution of alanine for serine at positions 14 and 15 and abolished the SstI site. Cre-mediated recombination removed the floxed neo cassette. (J:184548)

Generation of the Myl2^tm2.1Chen (Myl2^DM) allele

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myl2 Mutation: 22 strains or lines available

References

• Original: J:184548 Sheikh F, et al., Mouse and computational models link Mlc2v dephosphorylation to altered myosin kinetics in early cardiac disease. J Clin Invest. 2012 Apr 2;122(4):1209-21
• All: 2 reference(s)