C5ar1^tm1Song
Targeted Allele Detail

Summary

• Symbol: C5ar1^tm1Song
• Name: complement component 5a receptor 1; targeted mutation 1, Wen-Chao Song
• MGI ID: MGI:5426685
• Synonyms: GFP-KI⁺
• Gene: C5ar1 Location: Chr7:15980668-15993465 bp, - strand Genetic Position: Chr7, 8.77 cM
• Alliance: C5ar1^tm1Song page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184056
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 2. A cassette containing (5' to 3') an IRES, EGFP, SV40 polyadenlyation sequence, FRT-flanked neo cassette and loxP site was inserted into the 3' UTR. FACS analysis confirmed reduction of protein expression in elicited peritoneal neutrophils from homozygous mice. (J:184056)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C5ar1 Mutation: 31 strains or lines available

References

• Original: J:184056 Dunkelberger J, et al., C5aR expression in a novel GFP reporter gene knockin mouse: implications for the mechanism of action of C5aR signaling in T cell immunity. J Immunol. 2012 Apr 15;188(8):4032-42
• All: 1 reference(s)