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Nprl3tm1.1Drhi
Targeted Allele Detail
Summary
Symbol: Nprl3tm1.1Drhi
Name: nitrogen permease regulator-like 3; targeted mutation 1.1, Douglas R Higgs
MGI ID: MGI:5426855
Synonyms: deltaP6-
Gene: Nprl3  Location: Chr11:32181963-32217707 bp, - strand  Genetic Position: Chr11, 18.83 cM
Alliance: Nprl3tm1.1Drhi page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:184965
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Hypomorph, Modified regulatory region)
Mutations:    Insertion, Intergenic deletion
 
Mutation detailsA 2314 bp segment encoding Nprl3 promoter P6 (Rr246493) and exon 1 was replaced with a loxP site flanked neomycin resistance gene cassette. Subsequent cre-mediated recombination removed the neo cassette. RNA-FISH analysis indicates Nprl3 expression is eliminated in many tissues but not in erythroid cells. Expression in erythroid cells from homozygous mice is about 50% that of wild-type, presumably from alternatively spliced transcripts with alternative 1st exons. (J:184965)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 16 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nprl3 Mutation:  31 strains or lines available
References
Original:  J:184965 Kowalczyk MS, et al., Intragenic enhancers act as alternative promoters. Mol Cell. 2012 Feb 24;45(4):447-58
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory