Ift25^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ift25^{tm1b(EUCOMM)Wtsi}
• Name: intraflagellar transport 25; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5426875
• Synonyms: Ift25^null1
• Gene: Ift25 Location: Chr4:107110889-107137135 bp, + strand Genetic Position: Chr4, 50.18 cM
• Alliance: Ift25^{tm1b(EUCOMM)Wtsi} page
• IMPC: Ift25 gene page

Mutation origin

• Mutant Cell Line: EPD0039_5_G06
• Germline Transmission: Earliest citation of germline transmission: J:184749
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 106945715 of Chromosome 4 upstream of the critical exon(s) (Build 37). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 106946668. The critical exon(s) is/are thus flanked by loxP sites. Cre mediated recombination deleted exon 3. Western blot analysis confirmed the absence of protein expression in homozygous mice. (J:184749)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ift25 Mutation: 10 strains or lines available

References

• Original: J:184749 Keady BT, et al., IFT25 Links the Signal-Dependent Movement of Hedgehog Components to Intraflagellar Transport. Dev Cell. 2012 May 15;22(5):940-51
• All: 3 reference(s)