Polb^tm1.1Jbsw
Targeted Allele Detail

Summary

• Symbol: Polb^tm1.1Jbsw
• Name: polymerase (DNA directed), beta; targeted mutation 1.1, Joann B Sweasy
• MGI ID: MGI:5427084
• Synonyms: PolB^c, PolB^Y265C
• Gene: Polb Location: Chr8:23118142-23143451 bp, - strand Genetic Position: Chr8, 11.42 cM
• Alliance: Polb^tm1.1Jbsw page

Polb^tm1.1Jbsw/Polb^tm1.1Jbsw embryos are small

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183837
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv and C57BL/6

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: Nucleotides in exon 13 encoding tyrosine 265 codon were changed to encode cysteine and an FRT flanked neo cassette was inserted downstream of exon 13 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:183837)

Gene targeting of the Polb gene to generate the Polb^tm1.1Jbsw allele

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polb Mutation: 28 strains or lines available

References

• Original: J:183837 Senejani AG, et al., Y265C DNA polymerase beta knockin mice survive past birth and accumulate base excision repair intermediate substrates. Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6632-7
• All: 4 reference(s)