Pik3ca^tm1.1Waph
Targeted Allele Detail

Summary

• Symbol: Pik3ca^tm1.1Waph
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; targeted mutation 1.1, Wayne A Phillips
• MGI ID: MGI:5427579
• Synonyms: p110a^HR, Pik3ca^H1047R, Pik3ca^Lat-H1047R
• Gene: Pik3ca Location: Chr3:32451203-32520256 bp, + strand Genetic Position: Chr3, 15.7 cM, cytoband B
• Alliance: Pik3ca^tm1.1Waph page

Hyperproliferation of the ovarian surface epithelium in Pik3ca^tm1.1Waph/Pik3ca^tm1.1Waph mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184382
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

Allele Type: Targeted (Humanized sequence) Mutations: Insertion, Nucleotide substitutions Mutation details: Exon 20 was floxed. An addtional modified exon 20 was inserted downstream of the endogenous exon. The modified exon 20 contains nucleotide substitutions (CAT to AGG) that result in the amino acid substitution of arginine for histidine at position 1047 (H1047R). Flp-mediated recombination removed the FRT-flanked neo cassette. (J:184382)

Generation of the Pik3ca^tm1.1Waph allele

Disease models

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pik3ca^tm1.1Waph

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3ca Mutation: 66 strains or lines available

References

• Original: J:184382 Kinross KM, et al., An activating Pik3ca mutation coupled with Pten loss is sufficient to initiate ovarian tumorigenesis in mice. J Clin Invest. 2012 Feb 1;122(2):553-7
• All: 27 reference(s)