Wnk4^tm1Pfi
Targeted Allele Detail

Summary

• Symbol: Wnk4^tm1Pfi
• Name: WNK lysine deficient protein kinase 4; targeted mutation 1, Pfizer, Ltd
• MGI ID: MGI:5427702
• Gene: Wnk4 Location: Chr11:101151393-101168235 bp, + strand Genetic Position: Chr11, 64.51 cM
• Alliance: Wnk4^tm1Pfi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184790
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A genomic fragment, consisting of 75 bp 5' of exon 1, all of exon 1 and 737 bp 3' of exon 1, was replaced with a neo cassette via homologous recombination. Real time PCR and western blot analyses confirmed the absence of expression in homozygous mice. (J:184790)

Generation of the Wnk4^tm1Pfi allele

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wnk4 Mutation: 58 strains or lines available

Notes

ES cell line = TG-ES01-01 ESM07

References

• Original: J:184790 Castaneda-Bueno M, et al., Activation of the renal Na+:Cl- cotransporter by angiotensin II is a WNK4-dependent process. Proc Natl Acad Sci U S A. 2012 May 15;109(20):7929-34
• All: 12 reference(s)