Gata4^tm1Grg
Targeted Allele Detail

Summary

• Symbol: Gata4^tm1Grg
• Name: GATA binding protein 4; targeted mutation 1, Vidu Garg
• MGI ID: MGI:5427934
• Synonyms: Gata4 G295S^ki
• Gene: Gata4 Location: Chr14:63436371-63509141 bp, - strand Genetic Position: Chr14, 33.24 cM
• Alliance: Gata4^tm1Grg page

Growth retardation and a thin myocardium in Gata4^tm1Grg/Gata4^tm1Grg mice

Show the 9 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185124
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

Allele Type: Targeted (Humanized sequence, Hypomorph) Mutations: Insertion, Single point mutation Mutation details: A G to A mutation was introduced in exon 3 creating a glycine to serine substitution (G295S) and a floxed neo cassette was inserted downstream of exon 4 via homologous recombination. Immunoblot analysis indicated that protein levels in homozygous mice are similar to wild-type. (J:185124)

Targeting strategy for generation of Gata4^tm1Grg knock-in allele

Disease models

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gata4 Mutation: 36 strains or lines available

References

• Original: J:185124 Misra C, et al., Congenital heart disease-causing Gata4 mutation displays functional deficits in vivo. PLoS Genet. 2012 May;8(5):e1002690
• All: 2 reference(s)