Apc2^tm1Mno
Targeted Allele Detail

Summary

• Symbol: Apc2^tm1Mno
• Name: APC regulator of WNT signaling pathway 2; targeted mutation 1, Masaharu Noda
• MGI ID: MGI:5428211
• Gene: Apc2 Location: Chr10:80131811-80154097 bp, + strand Genetic Position: Chr10, 39.72 cM, cytoband B5-C2
• Alliance: Apc2^tm1Mno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:184856
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A construct containing an eGFP gene and an inverted neo cassette was inserted in frame in exon 2 after the initiation codon via homologous recombination. RT-PCR and western blot analysis confirmed the absence of expression in the brains of homozygous mice. EGFP expression was detected by RT-PCR and live fluorescent imaging in the nervous system and liver. (J:184856)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apc2 Mutation: 68 strains or lines available

References

• Original: J:184856 Shintani T, et al., Directional neuronal migration is impaired in mice lacking adenomatous polyposis coli 2. J Neurosci. 2012 May 9;32(19):6468-84
• All: 5 reference(s)