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Tg(Pklr-HRAS*G12V)23Ak
Transgene Detail
Summary
Symbol: Tg(Pklr-HRAS*G12V)23Ak
Name: transgene insertion 23, Axel Kahn
MGI ID: MGI:5428752
Synonyms: L-PK/H-ras
Transgene: Tg(Pklr-HRAS*G12V)23Ak  Location: unknown  
Alliance: Tg(Pklr-HRAS*G12V)23Ak page
Transgene
origin
Strain of Origin:  Not Specified
Transgene
description
Transgene Type:    Transgenic (Constitutively active, Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsThis transgene includes a 4.9-kb BamHI fragment containing the 4 exons of the human HRAS gene with a single amino acid substitution of a glycine to valine at codon 12 under the control of the regulatory regions of the rat L-type pyruvate kinase (1.46 kb of the 5' flanking sequence). In this construct, the distal activator sequence of Pklr located around position -3 kb is removed. The single amino-acid substitution produces a constitutively active product. The Pklr regulatory sequences confer positive transcriptional control by glucose so maximal expression of the transgene can be obtained under a carbohydrate-rich diet.Three lines were made, lines 11, 21, and 23. Twenty copies of the transgene were inserted into the genome in line 23 and Northern blot confirmed expression in small intestine and low levels in the liver. (J:44803)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:44803 Gilbert E, et al., In vivo effects of activated H-ras oncogene expressed in the liver and in urogenital tissues. Int J Cancer. 1997 Nov 27;73(5):749-56
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory