Arap3^tm1.2Sve
Targeted Allele Detail

Summary

• Symbol: Arap3^tm1.2Sve
• Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3; targeted mutation 1.2, Sonja Vermeren
• MGI ID: MGI:5428754
• Synonyms: Arap3^-
• Gene: Arap3 Location: Chr18:38105681-38132022 bp, - strand Genetic Position: Chr18, 19.85 cM
• Alliance: Arap3^tm1.2Sve page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185401
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted in intron 5 and an FRT flanked neo cassette and loxP site were inserted in intron 8 via homologous recombination. Flp mediated recombination removed the neo cassette. Cre mediated recombination removed exons 6 - 8. Western blot analysis confirmed the absence of protein expression in yolk sac derived endothelial cells. (J:185401)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arap3 Mutation: 61 strains or lines available

References

• Original: J:185401 Gambardella L, et al., PI3K signaling through the dual GTPase-activating protein ARAP3 is essential for developmental angiogenesis. Sci Signal. 2010;3(145):ra76
• All: 2 reference(s)