Tg(Pklr-HRAS*G12V)21Ak
Transgene Detail

Summary

• Symbol: Tg(Pklr-HRAS*G12V)21Ak
• Name: transgene insertion 21, Axel Kahn
• MGI ID: MGI:5428762
• Synonyms: L-PK/H-ras
• Transgene: Tg(Pklr-HRAS*G12V)21Ak Location: unknown
• Alliance: Tg(Pklr-HRAS*G12V)21Ak page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene includes a 4.9-kb BamHI fragment containing the 4 exons of the human HRAS gene with a single amino acid substitution of a glycine to valine at codon 12 under the control of the regulatory regions of the rat L-type pyruvate kinase (1.46 kb of the 5' flanking sequence). In this construct, the distal activator sequence of Pklr located around position -3 kb is removed. The single amino-acid substitution produces a constitutively active product. The Pklr regulatory sequences confer positive transcriptional control by glucose so maximal expression of the transgene can be obtained under a carbohydrate-rich diet. Three lines were made, lines 11, 21, and 23. Four copies of the transgene were inserted into the genome in line 21 and Northern blot confirmed expression in kidney, small intestine, epididymis, and thymus. (J:44803)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Phenotypic Similarity to Human Syndrome: Polycystic Kidney Disease (J:44803).

References

• Original: J:44803 Gilbert E, et al., In vivo effects of activated H-ras oncogene expressed in the liver and in urogenital tissues. Int J Cancer. 1997 Nov 27;73(5):749-56
• All: 1 reference(s)