Rdh10^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rdh10^{tm1d(KOMP)Wtsi}
• Name: retinol dehydrogenase 10 (all-trans); targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5429724
• Synonyms: Rdh10^delta
• Gene: Rdh10 Location: Chr1:16176106-16202774 bp, + strand Genetic Position: Chr1, 4.94 cM
• Alliance: Rdh10^{tm1d(KOMP)Wtsi} page
• IMPC: Rdh10 gene page

Mutation origin

• Mutant Cell Line: EPD0149_1_D08
• Germline Transmission: Earliest citation of germline transmission: J:185330
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 16097421 of Chromosome 1 upstream of exon 2 (Build 37). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 16098357. The critical exon(s) is/are thus flanked by loxP sites. Flp and Cre mediated recombination removed the lacZ and neo cassettes and exon 2. Quantitative RT-PCR indicates transcript levels are at about 50% that of wild-type in homozygous embryos. (J:148605, J:173534, J:185330)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rdh10 Mutation: 20 strains or lines available

Notes

J:185330 used EPD0149_1_D08 ES cells

References

• Original: J:185330 Sandell LL, et al., RDH10 oxidation of Vitamin A is a critical control step in synthesis of retinoic acid during mouse embryogenesis. PLoS One. 2012;7(2):e30698
• All: 11 reference(s)