Scn5a^{tm2.1(SCN5A)Rdn}
Targeted Allele Detail

Summary

• Symbol: Scn5a^{tm2.1(SCN5A)Rdn}
• Name: sodium channel, voltage-gated, type V, alpha; targeted mutation 2.1, Dan M Roden
• MGI ID: MGI:5430150
• Synonyms: CNS28^-
• Gene: Scn5a Location: Chr9:119312474-119408082 bp, - strand Genetic Position: Chr9, 71.33 cM
• Alliance: Scn5a^{tm2.1(SCN5A)Rdn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185686
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Recombinase mdiated cassette exchange (RMCE) replaced exon 2 with a loxP site, the human cDNA, an FRT-flanked hygro cassette and a loxP site. Flp-mediated recombination removed the hygro cassette. This knock-in lacks the conserved noncoding sequence 28 (CNS28) region contained in Scn5a^tm1Rdn. Western blot analysis confirmed increased protein expression in the heart compared to in Scn5a^tm1Rdn homozygotes. (J:185686)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scn5a Mutation: 105 strains or lines available

References

• Original: J:185686 Atack TC, et al., Informatic and functional approaches to identifying a regulatory region for the cardiac sodium channel. Circ Res. 2011 Jun 24;109(1):38-46
• All: 1 reference(s)