Gt(ROSA)26Sor^{tm2(Notch2)Ryn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(Notch2)Ryn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Ryuichi Nishinakamura
• MGI ID: MGI:5430391
• Synonyms: Gt(Rosa)26Sortm1(Notch2)Nis, Gt(ROSA)26Sor^{tm2(Notch2)Ryni}, R26Notch2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(Notch2)Ryn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185844
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(Notch2)Ryn} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Notch2 (MGI:97364)

• Mutation details: The floxed neo/STOP cassette and a flag-tagged Notch2 fragment, which encompassed amino acids 1701 to 2470 and lacked the transmembrane domain or S3 cleavage site but contained the intact transactivation domain and C-terminal PEST sequences, were knocked into the locus. (J:185844)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:185844 Fujimura S, et al., Notch2 activation in the embryonic kidney depletes nephron progenitors. J Am Soc Nephrol. 2010 May;21(5):803-10
• All: 5 reference(s)