Col1a1^{tm1(tetO-Yap1*)Lrsn}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-Yap1*)Lrsn}
• Name: collagen, type I, alpha 1; targeted mutation 1, Nils-Goran Larsson
• MGI ID: MGI:5430522
• Synonyms: tet-YAP^mut
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-Yap1*)Lrsn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185310
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Constitutively active, Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-Yap1*)Lrsn} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Yap1 (MGI:103262)

• Mutation details: RMCE on KH2 cells, which contain Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and a FRT-flanked PGK-Neo-pA in the 3' UTR of Col1a1, replaced the FRT-flanked neo cassette with a cassette that contained the tetracycline response element driving inducible expression of the mouse cDNA with nucleotide substitutions that result in the amino acid substitution of alanine for serine at position 127 (S127A). This mutation results in a constitutively active protein. (J:185310)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:185310 Jansson L, et al., Normal hematopoietic stem cell function in mice with enforced expression of the Hippo signaling effector YAP1. PLoS One. 2012;7(2):e32013
• All: 5 reference(s)