Ctnnb1^tm1.2Wvv
Targeted Allele Detail

Summary

• Symbol: Ctnnb1^tm1.2Wvv
• Name: catenin beta 1; targeted mutation 1.2, Wendy van Veelen
• MGI ID: MGI:5430598
• Synonyms: beta-catenin^E654
• Gene: Ctnnb1 Location: Chr9:120762466-120789573 bp, + strand Genetic Position: Chr9, 72.19 cM
• Alliance: Ctnnb1^tm1.2Wvv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185942
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 12 and a floxed FRT flanked PGK-neomycin selection cassette was inserted downstream of exon 15 followed by a duplication of exons 12-15. A tyrosine to glutamic acid codon change was incorporated at codon 654 of the duplicated exon 13. Correctly targeted mice were bred to "CAG-flp" mice to remove the selection cassette. Subsequent mating to "CAG-cre" mice substituted the mutated copy of exons 12-15 for the unmodified copy. (J:185942)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctnnb1 Mutation: 49 strains or lines available

References

• Original: J:185942 van Veelen W, et al., beta-catenin tyrosine 654 phosphorylation increases Wnt signalling and intestinal tumorigenesis. Gut. 2011 Sep;60(9):1204-12
• All: 2 reference(s)