Tas1r1^tm1Uboe
Targeted Allele Detail

Summary

• Symbol: Tas1r1^tm1Uboe
• Name: taste receptor, type 1, member 1; targeted mutation 1, Ulrich Boehm
• MGI ID: MGI:5430605
• Synonyms: Tas1r1^BLiR, Tas1r1-mCherry
• Gene: Tas1r1 Location: Chr4:152112371-152122947 bp, - strand Genetic Position: Chr4, 82.83 cM
• Alliance: Tas1r1^tm1Uboe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185286
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The open reading frame has been replaced with an mCherry expression cassette (transneuronal tracer truncated barley lectin, IRES, mCherry and self-excising neomycin resistance cassette). The final allele lacks the resistance cassette. Reporter expression is seen in single spindle-shaped cells of fungiform papillae and in seminiferous tubules in more mature round and elongated spermatids. Immunohistochemistry confirmed the absence of transcript expression in oral taste buds. (J:185286, J:207445)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tas1r1 Mutation: 27 strains or lines available

References

• Original: J:185286 Meyer D, et al., Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca(2)(+) and cAMP concentrations in spermatozoa. PLoS One. 2012;7(2):e32354
• All: 5 reference(s)