Ephb6/Trpv6^tm1.1Mfre
Targeted Allele Detail

Summary

• Symbol: Ephb6/Trpv6^tm1.1Mfre
• Name: transient receptor potential cation channel, subfamily V, member 6; targeted mutation 1.1, Marc Freichel
• MGI ID: MGI:5430762
• Synonyms: Trpv6^D541A, Trpv6^mt
• Gene: Trpv6 Location: Chr6:41597558-41613339 bp, - strand Genetic Position: Chr6, 19.79 cM, cytoband B2
• Alliance: Ephb6/Trpv6^tm1.1Mfre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185996
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: In the Trpv6 gene, a loxP site was inserted upstream of a modified exon 13 in which nucleotide substitutions (GAT to GCC) result in the amino acid substitution of aspartic acid with alanine at position 581 (p.D581A). An FRT site flanked neomycin resistance gene cassette with a 3' loxP site was inserted downstream of the last exon (15) into intron 16 of the Ephb6 gene on the opposite strand. Subsequent flp-mediated recombination removed the neo cassette, leaving a conditional-ready allele with exons 13-15 of Trpv6, as well as exons 17 and 18 of Ephb6, floxed. (J:185996)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trpv6 Mutation: 35 strains or lines available

References

• Original: J:185996 Weissgerber P, et al., Male fertility depends on Ca(2)+ absorption by TRPV6 in epididymal epithelia. Sci Signal. 2011;4(171):ra27
• All: 6 reference(s)