Zc3hc1^tm1.2Jduy
Targeted Allele Detail

Summary

• Symbol: Zc3hc1^tm1.2Jduy
• Name: zinc finger, C3HC type 1; targeted mutation 1.2, Justus Duyster
• MGI ID: MGI:5431129
• Gene: Zc3hc1 Location: Chr6:30366383-30391019 bp, - strand Genetic Position: Chr6, 12.52 cM
• Alliance: Zc3hc1^tm1.2Jduy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185584
• Parent Cell Line: CMT-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 2 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 1 and 2. RT-PCR and western blot analysis confirmed the absence of expression in homozygous MEFs and testicular cells. (J:185584)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zc3hc1 Mutation: 31 strains or lines available

References

• Original: J:185584 Illert AL, et al., Targeted inactivation of nuclear interaction partner of ALK disrupts meiotic prophase. Development. 2012 Jul;139(14):2523-34
• All: 2 reference(s)