Rnaseh2b^tm1Hgu
Targeted Allele Detail

Summary

• Symbol: Rnaseh2b^tm1Hgu
• Name: ribonuclease H2, subunit B; targeted mutation 1, MRC Human Genetics Unit
• MGI ID: MGI:5431529
• Synonyms: Rnaseh2b^E202X, Rnaseh2b^{tm1-hgu-A174T,E202X}
• Gene: Rnaseh2b Location: Chr14:62569517-62610445 bp, + strand Genetic Position: Chr14, 33.19 cM, cytoband C3
• Alliance: Rnaseh2b^tm1Hgu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186180
• Parent Cell Line: E14TG2aIV (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 7 was replaced with a modified exon 7 in which a G to A point mutation results in the amino acid substitution of threonine for alanine at position 174 (A174T). An additional mutation produces a G to T transversion resulting in a stop codon at position 202 (E202X). A floxed neo cassette was inserted upstream of the modified exon 7. Western blot analysis confirmed the absence of protein expression in E9.5 embryos. (J:186180)

Expression

In Mice Carrying this Mutation:	6 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnaseh2b Mutation: 43 strains or lines available

References

• Original: J:186180 Reijns MA, et al., Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development. Cell. 2012 May 25;149(5):1008-22
• All: 1 reference(s)