Crisp4^tm1(icre)Mpo
Targeted Allele Detail

Summary

• Symbol: Crisp4^tm1(icre)Mpo
• Name: cysteine-rich secretory protein 4; targeted mutation 1, Matti Poutanen
• MGI ID: MGI:5431546
• Synonyms: Crisp4 KI
• Gene: Crisp4 Location: Chr1:18185415-18216126 bp, - strand Genetic Position: Chr1, 5.65 cM, cytoband A4
• Alliance: Crisp4^tm1(icre)Mpo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185787
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Intragenic deletion
• Crisp4^tm1(icre)Mpo expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crisp4 (MGI:1925331)

• Mutation details: A construct containing a codon-improved cre (icre) recombinase-neomycin cassette was generated from a BAC containing the Crisp4 locus. The linearized vector targeted the third exon of Crisp4 in ES cells and homologys recombination resulted in a deletion causing a frameshift that disrupted endogenous gene expression. This allele expresses icre from the Crisp4 promoter, specifically in the epididymis. (J:185787)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crisp4 (mouse) Summary of all recombinase alleles driven by Crisp4. Alliance mouse Crisp4 gene page

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crisp4 Mutation: 21 strains or lines available

References

• Original: J:185787 Turunen HT, et al., Loss of cysteine-rich secretory protein 4 (Crisp4) leads to deficiency in sperm-zona pellucida interaction in mice. Biol Reprod. 2012 Jan;86(1):1-8
• All: 1 reference(s)