Tg(Nes-phiC31*/ERT2)2Imayo
Transgene Detail

Summary

• Symbol: Tg(Nes-phiC31*/ERT2)2Imayo
• Name: transgene insertion 2, Itaru Imayoshi
• MGI ID: MGI:5431595
• Synonyms: Nestin-PhiC31-ER^T2 line 2
• Transgene: Tg(Nes-phiC31*/ERT2)2Imayo Location: unknown
• Alliance: Tg(Nes-phiC31*/ERT2)2Imayo page

Transgene origin

• Strain of Origin: C57BL/6J or (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Nes-phiC31*/ERT2)2Imayo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Nes

• Mutation details: The construct was composed of a 5.8 kb fragment of the nestin promoter with sequences for optimized phiC31 recombinase (phiC31*; coding sequence in this construct lacks the nuclear localization signal) fused to the tamoxifen-inducible mutated estrogen receptor sequence (ERT2), followed by the 1.8 kb nestin second intron containing the neural stem/progenitor cell-specific enhancer. The SV40 polyadenylation sequence was also cloned into the vector. The purified construct was injected into fertilized one-cell embryos. 7 founders were established with 2 lines (#2 and 4) chosen for further analysis based on high transgene expression in the ventricular zone of the developing nervous system. (J:185547)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nes (species not specified) Summary of all recombinase alleles driven by Nes. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:185547 Imayoshi I, et al., In vivo evaluation of PhiC31 recombinase activity in transgenic mice. Neurosci Res. 2012 Jun;73(2):106-14
• All: 1 reference(s)