Tg(Nes-phiC31*)1Imayo
Transgene Detail

Summary

• Symbol: Tg(Nes-phiC31*)1Imayo
• Name: transgene insertion 1, Itaru Imayoshi
• MGI ID: MGI:5431596
• Synonyms: Nestin-PhiC31o
• Transgene: Tg(Nes-phiC31*)1Imayo Location: unknown
• Alliance: Tg(Nes-phiC31*)1Imayo page

Transgene origin

• Strain of Origin: C57BL/6J or (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Nes-phiC31*)1Imayo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Nes

• Mutation details: The construct was composed of a 5.8 kb fragment of the nestin promoter upstream of the sequence for optimized phiC31 recombinase (phiC31*), with the 1.8 kb second intron containing the neural stem/progenitor cell-specific enhancer downstream of the recombinase sequence. The SV40 polyadenylation sequence was also cloned into the vector. The linearized, purified construct was injected into pronuclei of fertilized one-cell embyros. Only one founder line was established. Mice show wide expression of phiC31 mRNA in the nervous system with no expression observed in non-neural tissues like somites. High expression is observed in the ventricular zone of the developing nervous system. (J:185547)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nes (species not specified) Summary of all recombinase alleles driven by Nes. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:185547 Imayoshi I, et al., In vivo evaluation of PhiC31 recombinase activity in transgenic mice. Neurosci Res. 2012 Jun;73(2):106-14
• All: 1 reference(s)