Tg(Th-phiC31*)1Imayo
Transgene Detail

Summary

• Symbol: Tg(Th-phiC31*)1Imayo
• Name: transgene insertion 1, Itaru Imayoshi
• MGI ID: MGI:5431597
• Synonyms: TH-PhiC31o
• Transgene: Tg(Th-phiC31*)1Imayo Location: unknown
• Alliance: Tg(Th-phiC31*)1Imayo page

Transgene origin

• Strain of Origin: C57BL/6J or (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Th-phiC31*)1Imayo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Th (25085)

  Th	(MGI:98735)

• Mutation details: The transgene construct has the optimized phiC31 sequence (phiC31*) downstream of the 9 kb fragment of the rat tyrosine hydroxylase (Th) promoter. A 0.75 kb downstream fragment of the mouse Th gene including the endogenous polyadenylation sequence was included after the phiC31 sequence. Only 1 founder was obtained. In offspring from crosses with R26attR lacz reporter mice, beta-galactosidase activity was observed in brain areas including the olfactory bulb, hypothalamus, midbrain, hindbrain and posterior hindbrain. Dopaminergic cell populations such as those in the substantia nigra, hypothalamus, and periglomerular region of the olfactory bulb and noradrenergic cells of the locus coeruleus showed staining. Some areas not expected to show Th activity such ans the accessory olfactory bulb stained, potentially due to ectopic Th promoter activity. (J:185547)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Th (rat) Summary of all recombinase alleles driven by Th. Comparative matrix view of recombinase activities Alliance rat Th gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:185547 Imayoshi I, et al., In vivo evaluation of PhiC31 recombinase activity in transgenic mice. Neurosci Res. 2012 Jun;73(2):106-14
• All: 1 reference(s)