Nuak2^tm1Sia
Targeted Allele Detail

Summary

• Symbol: Nuak2^tm1Sia
• Name: NUAK family, SNF1-like kinase, 2; targeted mutation 1, Shinichi Aizawa
• MGI ID: MGI:5431630
• Gene: Nuak2 Location: Chr1:132243864-132261226 bp, + strand Genetic Position: Chr1, 57.24 cM, cytoband E4
• Alliance: Nuak2^tm1Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:185597
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding domain of exon 1 was replaced with a floxed construct consisting of a lacZ gene and an FRT flanked neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of expression in homozygous embryos. (J:185597)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nuak2 Mutation: 32 strains or lines available

References

• Original: J:185597 Ohmura T, et al., Neural tube defects by NUAK1 and NUAK2 double mutation. Dev Dyn. 2012 Aug;241(8):1350-64
• All: 1 reference(s)