Tg(Nphs1-flpo)1Jhm
Transgene Detail

Summary

• Symbol: Tg(Nphs1-flpo)1Jhm
• Name: transgene insertion 1, Jeffrey H Miner
• MGI ID: MGI:5431870
• Synonyms: Nphs1-FLPo
• Transgene: Tg(Nphs1-flpo)1Jhm Location: unknown
• Alliance: Tg(Nphs1-flpo)1Jhm page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Nphs1-flpo)1Jhm expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nphs1 (MGI:1859637)

• Mutation details: A 4.2 kb fragment of the mouse Nphs1 (nephrin) promoter was cloned upstream of the optimized Flp recombinase (Flpo) with a 3' bovine growth hormone polyadenylation sequence. The construct was microinjected into fertilized pronuclei. 18 founders were obtained with 3 demonstrating excision of the Frt-flanked neo from the Lama5^neo allele. Flpo mRNA was detected by in situ hybridization in all three lines (1,3,5). Lines 1 and 3 when crossed to Lama5^tm3Jhm mice showed higher podocyte expression of FLPo and normal tubular basement membrane levels of laminin alpha-5 (Lama5). (J:185850)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nphs1 (mouse) Summary of all recombinase alleles driven by Nphs1. Alliance mouse Nphs1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:185850 Goldberg S, et al., Maintenance of glomerular filtration barrier integrity requires laminin alpha5. J Am Soc Nephrol. 2010 Apr;21(4):579-86
• All: 2 reference(s)