Gt(ROSA)26Sor^{tm2(EGFP/cre)Alj}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(EGFP/cre)Alj}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Alexandra L Joyner
• MGI ID: MGI:5432030
• Synonyms: R26^MASTR, Rosa26^{MASTR(frt-STOP-frt-GFPcre}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(EGFP/cre)Alj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186310
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(EGFP/cre)Alj} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gt(ROSA)26Sor (MGI:104735)

• Mutation details: A targeting vector was constructed to insert an SV40 splice acceptor sequence, an Frt-flanked PGK-neo cassette with polyA sequence, cDNA encoding an EGFP/cre fusion protein and 3 copies of the SV40 polyA signal into the first intron of Gt(ROSA)26Sor in ES cells. Flp expression will delete the neo cassette, permitting constitutive EGFP/cre expression in recombined cells and their progeny. In the absence of FLP recombinase, a very small percentage of cells may transiently expresses eGFPcre from the allele during embryonic development or in the adult. Furthermore, males spuriously express eGFPcre in their germline in the absence of FLP recombinase (although the eGFPcre protein is not transmitted to the fertilized egg). No eGFPcre expression is reported in the female germline prior to FLP recombination. (J:186310)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gt(ROSA)26Sor (mouse) Summary of all recombinase alleles driven by Gt(ROSA)26Sor. Alliance mouse Gt(ROSA)26Sor gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:186310 Lao Z, et al., MASTR: a technique for mosaic mutant analysis with spatial and temporal control of recombination using conditional floxed alleles in mice. Cell Rep. 2012 Aug 30;2(2):386-96
• All: 9 reference(s)