Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Alexandra L Joyner
• MGI ID: MGI:5432216
• Synonyms: Gt(ROSA)26Sor^{tm3(ACTB-FLPo/ERT2)Alj}, R26^FlpoER
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186310
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The sequence encoding a FLPo/ERT2 fusion protein (optimized FLP recombinase fused to the tamoxifen-responsive mutated human estrogen receptor ligand-binding domain), SV40 polyadenylation site and a loxP-flanked PGK-neo cassette was cloned downstream of the human CMV enhancer/chicken ACTB promoter (CAG). This construct was targeted to the Gt(ROSA)26Sor locus in embryonic stem cells. (J:186310)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

ES cell line = CY2.3 (129S6/SvEvTac-derived: gift of Rockefeller Gene Targeting Core)

References

• Original: J:186310 Lao Z, et al., MASTR: a technique for mosaic mutant analysis with spatial and temporal control of recombination using conditional floxed alleles in mice. Cell Rep. 2012 Aug 30;2(2):386-96
• All: 16 reference(s)