Tg(Nphs1-cre/EGFP)1Crjk
Transgene Detail

Summary

• Symbol: Tg(Nphs1-cre/EGFP)1Crjk
• Name: transgene insertion 1, Chris Kennedy
• MGI ID: MGI:5432567
• Synonyms: CreEGFP^pod+
• Transgene: Tg(Nphs1-cre/EGFP)1Crjk Location: unknown
• Alliance: Tg(Nphs1-cre/EGFP)1Crjk page

Transgene origin

• Strain of Origin: C57BL/6J x C3H/HeJ

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Nphs1-cre/EGFP)1Crjk expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Nphs1

• Mutation details: An 8.3 kb fragment of the Nphs1 promoter was used to drive podocyte specific cre/EGFP expression in the construct. Three founders were obtained. EGFP immunohistochemistry showed protein expression was restricted to podocytes and was absent from other tissues. When founders were crossed to ROSA26 reporter mice, staining was observed extensively in the glomeruli in all mice (with weak staining observed in the brain, but not in other tissues). A representative line 1 was entered. Mice were backcrossed 10 generations onto FVB/N. (J:185912)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nphs1 (species not specified) Summary of all recombinase alleles driven by Nphs1.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:185912 Stitt-Cavanagh EM, et al., A maladaptive role for EP4 receptors in podocytes. J Am Soc Nephrol. 2010 Oct;21(10):1678-90
• All: 1 reference(s)