Rasgrp4^{tm1d(KOMP)Lste}
Targeted Allele Detail

Summary

• Symbol: Rasgrp4^{tm1d(KOMP)Lste}
• Name: RAS guanyl releasing protein 4; targeted mutation 1d, Len Stephens
• MGI ID: MGI:5432886
• Gene: Rasgrp4 Location: Chr7:28834358-28853386 bp, + strand Genetic Position: Chr7, 16.94 cM, cytoband B1
• Alliance: Rasgrp4^{tm1d(KOMP)Lste} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186412
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The recombinase site flanked reporter cassette (FRT site, engrail2 splice acceptor, IRES, LacZ, polyadenylation sequence and loxP site) and selection cassettes (beta-geo, polyadenylation site, FRT site and loxP site) were inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 6. Flp mediated recombination removed the reporter and selection cassettes. Cre-mediated recombination removed exons 5 and 6, which encode the catalytic domain. (J:186412)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rasgrp4 Mutation: 41 strains or lines available

References

• Original: J:186412 Suire S, et al., GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4. EMBO J. 2012 Jul 18;31(14):3118-29
• All: 3 reference(s)