Axin2^{tm1(cre/ERT2)Rnu}
Targeted Allele Detail

Summary

• Symbol: Axin2^{tm1(cre/ERT2)Rnu}
• Name: axin 2; targeted mutation 1, Roel Nusse
• MGI ID: MGI:5433373
• Synonyms: Axin2^creERT2
• Gene: Axin2 Location: Chr11:108811175-108841609 bp, + strand Genetic Position: Chr11, 71.83 cM
• Alliance: Axin2^{tm1(cre/ERT2)Rnu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186834
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intergenic deletion
• Axin2^{tm1(cre/ERT2)Rnu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Axin2 (MGI:1270862)

• Mutation details: A cre/ERT2 sequence (cre recombinase fused to a G400V/M543A/L544A triple mutation ofthe human estrogen receptor ligand binding domain) and an FRT-flanked puromycin resistance cassette were knocked into exon 2 of the Axin2 gene. Transient flp expression in ES cells and crosses to flp-expressing strains were unsuccessful at excising the selection cassette, but its presence does not seem to have negative side effects. (J:74286, J:186834)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Axin2 (mouse) Summary of all recombinase alleles driven by Axin2. Comparative matrix view of recombinase activities Alliance mouse Axin2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Axin2 Mutation: 44 strains or lines available

References

• Original: J:186834 van Amerongen R, et al., Developmental Stage and Time Dictate the Fate of Wnt/beta-Catenin-Responsive Stem Cells in the Mammary Gland. Cell Stem Cell. 2012 Sep 7;11(3):387-400
• All: 57 reference(s)