Lrp1^tm5Ajmr
Targeted Allele Detail

Summary

• Symbol: Lrp1^tm5Ajmr
• Name: low density lipoprotein receptor-related protein 1; targeted mutation 5, Anton J M Roebroek
• MGI ID: MGI:5433527
• Synonyms: n1 + n2
• Gene: Lrp1 Location: Chr10:127374030-127457017 bp, - strand Genetic Position: Chr10, 74.52 cM, cytoband B2-D1
• Alliance: Lrp1^tm5Ajmr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186908
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (RMCE-ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Lrp1 knock-in mouse carrying mutant Lrp1 cDNA sequences in the 3' end of the gene replacing the corresponding genomic sequences: no intron 76 to intron 88 sequences present. The mutations are introduced to inactivate the proximal NPXY (NPXY1) motif (NPTY - AATA) and the distal NPXYXXL (NPXY2) motif (NPVYATL - AAVAATL) in the intracellular domain of LRP1. The Lrp1 knock-in allele was generated by application of recombinase mediated cassette exchange (RMCE) in a ES cell line carrying an exchangeable cassette with a selection marker in the Lrp1 gene replacing the 3' end exons (exons 76 to 89) of the gene. By application of RMCE the Lrp1 gene was functionally restored by wild-type or mutant Lrp1 cDNA sequences resulting in a series of Lrp1 knock-in mutant alleles and mice including the Lrp1 proximal NPXY (NPXY1) motif and distal NPXYXXL (NPXY2) motif double knock-in mutant. (J:186908)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lrp1 Mutation: 211 strains or lines available

References

• Original: J:186908 Reekmans SM, et al., Inactivation of the proximal NPXY motif impairs early steps in LRP1 biosynthesis. Cell Mol Life Sci. 2010 Jan;67(1):135-45
• All: 1 reference(s)