Krt17^tm1(cre)Murr
Targeted Allele Detail

Summary

• Symbol: Krt17^tm1(cre)Murr
• Name: keratin 17; targeted mutation 1, Steve Murray
• MGI ID: MGI:5435552
• Synonyms: Krt17^{tm1(cre,Cerulean)Murr}
• Gene: Krt17 Location: Chr11:100147043-100151855 bp, - strand Genetic Position: Chr11, 63.44 cM
• Alliance: Krt17^tm1(cre)Murr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187143
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Krt17^tm1(cre)Murr expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Krt17 (MGI:96691)

• Mutation details: A targeting vector was designed to replace the stop codon of the endogenous keratin 17 (Krt17) gene with (from 5' to 3') a viral 2A oligopeptide (to mediate ribosomal skipping), cre recombinase, another 2A oligopeptide, the cerulean variant of green fluorescent protein (GFP), and a bovine polyadenylation sequence. This sequence was followed by a frt-flanked neomycin (neo) resistance cassette. This construct was electroporated into C57BL/6NTac derived JM8A3 embryonic stem (ES) cells. (J:187143)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Krt17 (mouse) Summary of all recombinase alleles driven by Krt17. Comparative matrix view of recombinase activities Alliance mouse Krt17 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Krt17 Mutation: 28 strains or lines available

References

• Original: J:187143 Murray S, Generation of a keratin 17 cre/cerulean knock-in allele. MGI Direct Data Submission. 2012
• All: 1 reference(s)