Etl4^tm1(cre)Kymm
Targeted Allele Detail

Summary

• Symbol: Etl4^tm1(cre)Kymm
• Name: enhancer trap locus 4; targeted mutation 1, Ken-ichi Yamamura
• MGI ID: MGI:5435947
• Synonyms: Skt^cre
• Gene: Etl4 Location: Chr2:19915018-20815346 bp, + strand Genetic Position: Chr2, 14.27 cM, cytoband A3
• Alliance: Etl4^tm1(cre)Kymm page

Mutation origin

• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (RMCE-ready, Recombinase)
• Mutation: Insertion
• Etl4^tm1(cre)Kymm expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Etl4 (MGI:95454)

• Mutation details: Cre recombinase was inserted into intron 14 of the Etl4 (Skt) locus by recombinase mediated cassette exchange using an exchangeable gene trap system using left element/right element (LE/RE) mutant lox sites. The IRES-cre sequence was exchanged with the IRES-lacZ-neo unit in the original trap vector by co-electroporation of the vector and pCAGGS-cre into TT2 ES cells containing the trapped Etl4 gene. (J:187217)

Generation of the Etl4^tm1(cre)Kymm allele.

Recombinase activity

Activity:	Tissue activity of this recombinase allele MGI has not yet included tissue activity data for this allele in any anatomical systems.
Driver:	Etl4 (mouse) Summary of all recombinase alleles driven by Etl4. Alliance mouse Etl4 gene page

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Etl4^tm1(cre)Kymm/Etl4^+ Tg(CAG-EGFP-CAT-DTA)2Masa/0	involves: C3H * C57BL/6NCrlj * CBA/JNCrlj

Phenotypes:

Affected Systems	cn1
show or hide all annotated terms
behavior/neurological	N
behavior/neurological phenotype	N
mortality/aging	√
preweaning lethality	√
skeleton	√
abnormal nucleus pulposus morphology	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Etl4 Mutation: 485 strains or lines available

References

• Original: J:187217 Abe K, et al., A Cre knock-in mouse line on the Sickle tail locus induces recombination in the notochord and intervertebral disks. Genesis. 2012 Oct;50(10):758-65
• All: 1 reference(s)