Calca^{tm1.1(EGFP/HBEGF)Mjz}
Targeted Allele Detail

Summary

• Symbol: Calca^{tm1.1(EGFP/HBEGF)Mjz}
• Name: calcitonin/calcitonin-related polypeptide, alpha; targeted mutation 1.1, Mark Zylka
• MGI ID: MGI:5437493
• Synonyms: CGRPalpha-GFP
• Gene: Calca Location: Chr7:114230713-114235592 bp, - strand Genetic Position: Chr7, 59.99 cM
• Alliance: Calca^{tm1.1(EGFP/HBEGF)Mjz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187267
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/Sv

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Calca^{tm1.1(EGFP/HBEGF)Mjz} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Mutation details: An FRT-flanked neo cassette, floxed farnesylated EGFP and human diphtheria toxin receptor (HBEGF/DTR) were inserted at the translation initiation site. Flp-mediated recombination removed the neo cassette and allowed for the expression of the GFP and HBEGF. (J:187267)

Schematic of the Calca^{tm1.1(EGFP/HBEGF)Mjz} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Calca Mutation: 23 strains or lines available

References

• Original: J:187267 McCoy ES, et al., CGRPalpha-expressing sensory neurons respond to stimuli that evoke sensations of pain and itch. PLoS One. 2012;7(5):e36355
• All: 22 reference(s)