Col1a1^{tm1(tetO-Deptor)Dmsa}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-Deptor)Dmsa}
• Name: collagen, type I, alpha 1; targeted mutation 1, David M Sabatini
• MGI ID: MGI:5437855
• Synonyms: iDeptor
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-Deptor)Dmsa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187378
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-Deptor)Dmsa} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Deptor (MGI:2146322)

• Col1a1^{tm1(tetO-Deptor)Dmsa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: Recombinase mediated cassette exchange in KH2 ES cells replaced the existing cassette at this locus with one encoding a tetO minimal promoter and Deptor coding sequence. (J:187378)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:187378 Laplante M, et al., DEPTOR Cell-Autonomously Promotes Adipogenesis, and Its Expression Is Associated with Obesity. Cell Metab. 2012 Aug 8;16(2):202-12
• All: 2 reference(s)