Actb^tm1Itl
Targeted Allele Detail

Summary

• Symbol: Actb^tm1Itl
• Name: actin, beta; targeted mutation 1, inGenious Targeting Laboratory
• MGI ID: MGI:5438548
• Synonyms: Actb^M
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^tm1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187804
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Twenty-four copies of the MS2 binding site (MBS) for the bacteriophage MS2 capsid protein and a floxed neo cassette were introduced into the 3' UTR. The MBS copies increase the signal-to-noise ratio of the mRNA. (J:187804)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

References

• Original: J:187804 Lionnet T, et al., A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nat Methods. 2011 Feb;8(2):165-70
• All: 4 reference(s)